porcine spinal cords  (Tissue Source LLC)

Journal: The Journal of Comparative Neurology

Article Title: Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex

doi: 10.1002/cne.24409

Figure Lengend Snippet: Information of primary antibodies

Article Snippet: Glial fibrillary acidic protein (GFAP) , GFAP purified from porcine spinal cord , Millipore; mouse monoclonal; Cat# MAB360, RRID: AB_2109815 (Clone GA5) , 1:4,000.

Techniques: Purification

Journal: The Journal of comparative neurology

Article Title: Sex and Laterality Differences in Medial Amygdala Neurons and Astrocytes of Adult Mice

doi: 10.1002/cne.23964

Figure Lengend Snippet: List of Antibodies Used for GFAP Immimohistochemistry

Article Snippet: In western blots of mouse brain lysate, the primary antiserum recognizes a single band at ~51 kDa, corresponding to GFAP (manufacturer information). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antigen Description of immunogen Source, host species, cat. #, clone or lot #, RRID Concentration used Glial fibrillary acidic protein clone GA5 Purified porcine spinal cord glial fibrillary acidic protein EMD Millipore, mouse monoclonal, Cat# MAB360, RRID:AB_2109815 0.02 μl/ml Antimouse IgG Mouse IgG (H+L), rat absorbed, biotinylated Vector Laboratories, horse polyclonal, Cat# BA-2001, RRID:AB_2336180 0.4 μl/ml Open in a separate window List of Antibodies Used for GFAP Immimohistochemistry Nissl stain The other series of sections were brought to room temperate, rinsed in PBS-GT, and mounted onto gelsubbed slides.

Techniques: Concentration Assay, Purification, Plasmid Preparation

Journal: The Journal of comparative neurology

Article Title: Sex and Laterality Differences in Medial Amygdala Neurons and Astrocytes of Adult Mice

doi: 10.1002/cne.23964

Figure Lengend Snippet: The adult MePD in adult male and female mice was stained for the astrocytic protein, glial fibrillary acidic protein (GFAP) and counterstained with hematoxylin, or stained for Nissl substance using thionin. The most rostral portions (A) are easily discernible in GFAP+hematoxylin (B) and Nissl (C)-stained tissues. Marked sex differences in the cross-sectional area of the MePD are revealed in representative sections from middle portion of the MePD (D–H). The caudal-most portion of the MePD (I) is also clearly visible in sections stained for either GFAP (J) or Nissl (K). GFAP+hematoxylin-stained tissue (L) was used to analyze astrocyte (white arrowheads) number and process complexity of astrocytes. Nissl-stained tissue (M) was used to quantify total glia (black arrows) and neurons (black arrowheads) in the MePD of adult male and female mice.

Article Snippet: In western blots of mouse brain lysate, the primary antiserum recognizes a single band at ~51 kDa, corresponding to GFAP (manufacturer information). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antigen Description of immunogen Source, host species, cat. #, clone or lot #, RRID Concentration used Glial fibrillary acidic protein clone GA5 Purified porcine spinal cord glial fibrillary acidic protein EMD Millipore, mouse monoclonal, Cat# MAB360, RRID:AB_2109815 0.02 μl/ml Antimouse IgG Mouse IgG (H+L), rat absorbed, biotinylated Vector Laboratories, horse polyclonal, Cat# BA-2001, RRID:AB_2336180 0.4 μl/ml Open in a separate window List of Antibodies Used for GFAP Immimohistochemistry Nissl stain The other series of sections were brought to room temperate, rinsed in PBS-GT, and mounted onto gelsubbed slides.

Techniques: Staining

Journal: The Journal of comparative neurology

Article Title: Sex and Laterality Differences in Medial Amygdala Neurons and Astrocytes of Adult Mice

doi: 10.1002/cne.23964

Figure Lengend Snippet: Regional volume of the MePD in adult C57Bl/6J mice is sexually dimorphic. Males have a larger MePD than females as defined by either (A) GFAP (counterstained with hematoxylin) or (B) Nissl staining. The MePD is also significantly larger on the left than the right when collapsed across sex in Nissl-stained sections.

Article Snippet: In western blots of mouse brain lysate, the primary antiserum recognizes a single band at ~51 kDa, corresponding to GFAP (manufacturer information). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antigen Description of immunogen Source, host species, cat. #, clone or lot #, RRID Concentration used Glial fibrillary acidic protein clone GA5 Purified porcine spinal cord glial fibrillary acidic protein EMD Millipore, mouse monoclonal, Cat# MAB360, RRID:AB_2109815 0.02 μl/ml Antimouse IgG Mouse IgG (H+L), rat absorbed, biotinylated Vector Laboratories, horse polyclonal, Cat# BA-2001, RRID:AB_2336180 0.4 μl/ml Open in a separate window List of Antibodies Used for GFAP Immimohistochemistry Nissl stain The other series of sections were brought to room temperate, rinsed in PBS-GT, and mounted onto gelsubbed slides.

Techniques: Staining

Journal: The Journal of comparative neurology

Article Title: Sex and Laterality Differences in Medial Amygdala Neurons and Astrocytes of Adult Mice

doi: 10.1002/cne.23964

Figure Lengend Snippet: Schematic summarizing sex and laterality differences in MePD cytoarchitecture in adult rats and mice. Neuron and glial cell number was measured in Nissl-stained sections, whereas number and arbor complexity of astrocytes were measured in GFAP-stained sections. Schematic is based on data reported in Cooke et al., 1999, 2003; Johnson et al., 2008, 2012, 2013; Morris et al., 2008a,b.

Article Snippet: In western blots of mouse brain lysate, the primary antiserum recognizes a single band at ~51 kDa, corresponding to GFAP (manufacturer information). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antigen Description of immunogen Source, host species, cat. #, clone or lot #, RRID Concentration used Glial fibrillary acidic protein clone GA5 Purified porcine spinal cord glial fibrillary acidic protein EMD Millipore, mouse monoclonal, Cat# MAB360, RRID:AB_2109815 0.02 μl/ml Antimouse IgG Mouse IgG (H+L), rat absorbed, biotinylated Vector Laboratories, horse polyclonal, Cat# BA-2001, RRID:AB_2336180 0.4 μl/ml Open in a separate window List of Antibodies Used for GFAP Immimohistochemistry Nissl stain The other series of sections were brought to room temperate, rinsed in PBS-GT, and mounted onto gelsubbed slides.

Techniques: Staining